FIG. 8.

ELISA analysis of binding of fH to BBL39 and BBN38 peptides. Wells of a 96-well microtiter plate were coated with peptides or appropriate controls as indicated. All procedures are described in the text. Data are averages of three separate determinations. Binding of peptides and r proteins to the wells was assessed by using anti-OspE antisera (solid bars). The abilities of the peptides to bind infection serum Abs were assessed by using anti-B. burgdorferi B31MIpc infection sera (hatched bars). Abilities to bind hfH were assessed by using anti-fH antisera (open bars).