TABLE 1.
Oligonucleotide primers
| Primer | Sequence (5′ to 3′) |
|---|---|
| UHB(+) | GTTGTTTAAAAATTACATTTGCG |
| L40-R(−) | TATTGTTTTCTTCTACACCCTT |
| N38-UHB(+) | GTAGTCTTTTGTAATGAGCCGG |
| N39-R2(−) | CATTTAATTTAGTTCTTAGCGCGT |
| UHB-N38(+) | GTTGGTTAAAATTACATTTGAG |
| N39-R1(−) | CTTCTTTTTCTTCTTCTTG |
| L39-1(+) | GACGACGACAAGATGGAGAAATTTATGAATAAG |
| L39-16(+) | GACGACGACAAGATGCTTATAGGTGCTTGCAAG |
| L39-54(+) | GACGACGACAAGATGAGTAATAACTGGGCAGAC |
| L39-77(+) | GACGACGACAAGATGAACGCTGGGGGACATTC |
| L39-99(+) | GACGACGACAAGATGGCAATGACTGAAGGTGGA |
| L39-125(+) | GACGACGACAAGATGAAGAATAAAGAGATAAAAACAAAG |
| L39-82(−) | GAGGAGAAGCCCGGTTTACGAATGTCCCCCAGCG |
| L39-104(−) | GAGGAGAAGCCCGGTTTATCCACCTTCAGTCATTGC |
| L39-132(−) | GAGGAGAAGCCCGGTTTACTTTGTTTTTATCTCTTTATTC |
| L39-173(−) | GAGGAGAAGCCCGGTTTATTTTAAATTTCTTTTAAGCTC |
| N38-16(+) | GACGACGACAAGATGCTTATAGGTGCTTGCAAAATTC |
| N38-67(+) | GACGACGACAAGATGAATAGTAACTGGACAGAC |
| N38-90(+) | GACGACGACAAGATGAACGCTGGGGGACATTC |
| N38-112(+) | GACGACGACAAGATGGCAATGACTAAAGGCGG |
| N38-136(+) | GACGACGACAAGATGGGTATCCAAAACAAAGAGATC |
| N38-186(−) | GAGGAGAAGCCCGGTTTATTTTAAATTTTTTTTAAGCAC |
| N38-142(−) | GAGGAGAAGCCCGGTTTAGATCTCTTTGTTTTGGATACC |
| N38-117(−) | GAGGAGAAGCCCGGTTTATCCGCCTTTAGTCATTGC |
| N38-95(−) | GAGGAGAAGCCCGGTTTACGAATGTCCCCCAGCG |
| O39-F1(+) | GACGACGACAAGATTTATGCAAGTGGTGAAAATCTA |
| O39-R3(−) | GAGGAGAAGCCCGGTTGTTATTCTTTTTTATCTTCTTCTATTCC |
| R42-F1(+) | GACGACGACAAGATTGATGTAACTAGTAAAGATTTA |
| R42-R3(−) | GAGGAGAAGCCCGGTCTTTATTCTTTTTTACCTTCTACAG |
a
Underlining indicates the tail sequences incorporated into each primer to allow for annealing with LIC vectors as described in the text.