Fig. 2.

pAEA is an intermediate of AEA synthesis. (a) MS/MS spectra of AEA, pAEA, and d₄AEA, the internal standard. (b) Representative ion chromatograms for pAEA and AEA obtained from ≈400 fmol standards (Upper) and RAW 264.7 (Lower) cells treated with NaVO₃ and LPS by using SRM. The transitions of m/z 348 → 287, m/z 352 → 287, and m/z 428 → 330 were selected for AEA, d₄AEA, and pAEA, respectively. (c) Effect of NaVO₃ on pAEA levels in brain tissue extract. (Left) Representative SRM ion chromatograms obtained from the control brain sample. The presence of pAEA peak at 4 min was further confirmed by spiking with standard pAEA. (Right) The peak area ratio of pAEA to d₄AEA in brain samples incubated with or without NaVO₃ (mean ± SE, n = 3).