Figure 3.

The effects of JV-1–36 (300 nM) (A) and JV-1–53 (300 nM) (B) on basal and VIP-induced PRL release from rat pituitary cells in a superfusion system. After two 3-min infusions of 10 nM VIP (solid filled bars), the cells were exposed to JV-1–36 or JV-1–53 for 9 min (checkered bars) followed by a simultaneous infusion of these analogs and 10 nM VIP for an additional 3 min (filled bar) (0-min inhibition). The duration of the inhibitory effect of these analogs was evaluated by three consecutive 3-min infusions of 10 nM VIP at 30-min intervals (filled bars) (30-, 60-, and 90-min inhibition). The second VIP-induced PRL response before the antagonist exposure was used as reference value.