Table 3.
Primers used in this study
| Primer | Sequence of primers | Comments | Product size (bp) |
| 2pbadFOR | 5'-CACCATGGCCAAGTTCTTTATC-3' | Sense primer for the cloning of orf2-coding into pBAD202 | 3192 |
| 2pbadREV | 5'-TTACTGCCCCTTCGAATCGTTT-3' | Antiense primer for the cloning of orf2-coding into pBAD202 | |
| 1rtFOR | 5'-CCACGCCGTTCGTTCGCATCT-3' | Sense primer for RT-PCR analysis of orf1 | 577 |
| 1rtREV | 5'-GCTTGGCGAACCACCGGTTGA-3' | Antisense primer for RT-PCR analysis orf1 | |
| 2rtFOR | 5'-CGAAGCTGAAGCCGTTGAC-3' | Sense primer for RT-PCR analysis orf2 | 511 |
| 2rtREV | 5'-TCGTGCTGTCGGCGGTGTT-3' | Antisense primer for RT-PCR analysis orf2 | |
| 3rtFOR | 5'-CGCGCTCACCGACCTGATGTT-3' | Sense primer for RT-PCR analysis orf3 | 531 |
| 3rtREV | 5'-AGCGCGAGGAACACGACGAGC-3' | Antisense primer for RT-PCR analysis orf3 | |
| 4rtFOR | 5'-AACATCCTGCGCTGAAGGTCAAC-3' | Sense primer for RT-PCR analysis orf4 | 537 |
| 4rtREV | 5'-TGACAGCCCCTCTTCCGCCATCAC-3' | Antisense primer for RT-PCR analysis orf4 | |
| 5rtFOR | 5'-TACGTGCTGCGCGAACTGAAG-3' | Sense primer for RT-PCR analysis orf5 | 594 |
| 5rtREV | 5'-AACAGCCACATCACGCAAAACAC-3' | Antisense primer for RT-PCR analysis orf5 | |
| 6rtFOR | 5'-TCGAATGGGTGCTGAACCGC-3' | Sense primer for RT-PCR analysis orf6 | 477 |
| 6rtREV | 5'-CGAATTCTTCCGAGTTGGGGCT-3' | Antisense primer for RT-PCR analysis orf6 | |
| 8rtFOR | 5'-CAAGGTCGTGACCGGCGTGTA-3' | Sense primer for RT-PCR analysis orf8 | 517 |
| 8rtREV | 5'-GATCATCGACAGCACCATCAGC-3' | Antisense primer for RT-PCR analysis orf8 | |
| 9rtFOR | 5'-CTATTCGATCAATACGCTCACG-3' | Sense primer for RT-PCR analysis orf9 | 493 |
| 9rtREV | 5'-CGCATAGACGGCGAACACCAC-3' | Antisense primer for RT-PCR analysis orf9 | |
| 10rtFOR | 5'-AACTCGCTGCAGATCTCGGAC-3' | Sense primer for RT-PCR analysis orf10 | 521 |
| 10rtREV | 5'-ATCGCGAACTGCTTGTAGAACTG-3' | Antisense primer for RT-PCR analysis orf10 | |
| 11rtFOR | 5'-TCGCGGCGAAGATGGAGGAC-3' | Sense primer for RT-PCR analysis orf11 | 522 |
| 11rtREV | 5'-TCGTCGCCATCGGATGGAACAT-3' | Antisense primer for RT-PCR analysis orf11 | |
| 12rtFOR | 5'-CGCGCTCAACGACTGGTTCCT-3' | Sense primer for RT-PCR analysis orf12 | 520 |
| 12rtREV | 5'-CAGTTGCGATCGGTCGTAGGTC-3' | Antisense primer for RT-PCR analysis orf12 | |
| 13rtFOR | 5'-CAGGTTACACAGATGCGAACGA-3' | Sense primer for RT-PCR analysis orf13 | 530 |
| 13rtREV | 5'-AAGCCCGACACGTGACGAGCG-3' | Antisense primer for RT-PCR analysis orf13 | |
| 14rtFOR | 5'-ACGCCGACCGACATCTTTCCG-3' | Sense primer for RT-PCR analysis orf14 | 615 |
| 14rtREV | 5'-CTGCGTGCTGCCGTTCATCTGC-3' | Antisense primer for RT-PCR analysis orf14 | |
| 15rtFOR | 5'-AGAGTTTGATCATGGCTCAG-3' | Sense primer for RT-PCR analysis rRNA 16S gene | 1490 |
| 16rtREV | 5'-TACCTTGTTACGACTTCAC-3' | Antisense primer for RT-PCR analysis rRNA 16S gene |