FIG. 1.

Inhibition of Salmonella growth by acidified nitrite. (A) Typical growth curve. One millimolar nitrite significantly slows growth of Salmonella, and 10 mM nitrite is slightly bactericidal. Error bars represent standard error (n = 5). OD₆₃₀, optical density at 630 nm. (B) Typical pH curve starting in LB broth, pH 5. The unbuffered media change pH over the time of the experiment but not dramatically. Typical pH values ranged from 5.3 to 5.5 at the time of nitrite addition and from 5.4 to 5.6 after 8 h of standing incubation.