TABLE 1.
STEC strain | Serotype | Phage type | Phage titer/ml of culture filtrate | No. of plaques probed | No. of plaques hybridizing to: |
|
---|---|---|---|---|---|---|
Stx1 probe | Stx2 probe | |||||
CF16 | NDc | ND | 8.3 × 102 | 332 | 0 | 0 |
E45040 | 1.2 × 106 | 121 | 0 | 121 | ||
E45040 (UV)b | O111:H− | ND | 6.7 × 108 | 670 | 0 | 670 |
E83819 | 1.7 × 106 | 171 | 171 | 0 | ||
E83819 (UV) | O157:H7 | 14 | 1.6 × 108 | 160 | 160 | 0 |
E85539 | O157:H7 | 4 | 1.4 × 106 | 175 | 0 | 175 |
E86654 | O157:H7 | 4 | 7.1 × 104 | 126 | 121 | 5 |
PS14 | 1.5 × 106 | 510 | 0 | 510 | ||
PS14 (UV) | O157H7 | ND | 2.4 × 107 | 240 | 0 | 240 |
Plaques were probed with oligonucleotides specific to Stx1 and Stx2 toxin genes. Data for serotypes and phage types were obtained from the CPHL.
(UV), culture was subjected to UV irradiation to induce phage production.
ND, not determined.