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. 2003 Jun;71(6):3409–3418. doi: 10.1128/IAI.71.6.3409-3418.2003

TABLE 1.

Oligonucleotide probing of plaques on an MC1061 bacterial lawn infected with culture filtrates from various STEC strainsa

STEC strain Serotype Phage type Phage titer/ml of culture filtrate No. of plaques probed No. of plaques hybridizing to:
Stx1 probe Stx2 probe
CF16 NDc ND 8.3 × 102 332 0 0
E45040 1.2 × 106 121 0 121
E45040 (UV)b O111:H− ND 6.7 × 108 670 0 670
E83819 1.7 × 106 171 171 0
E83819 (UV) O157:H7 14 1.6 × 108 160 160 0
E85539 O157:H7 4 1.4 × 106 175 0 175
E86654 O157:H7 4 7.1 × 104 126 121 5
PS14 1.5 × 106 510 0 510
PS14 (UV) O157H7 ND 2.4 × 107 240 0 240
a

Plaques were probed with oligonucleotides specific to Stx1 and Stx2 toxin genes. Data for serotypes and phage types were obtained from the CPHL.

b

(UV), culture was subjected to UV irradiation to induce phage production.

c

ND, not determined.