TABLE 2.

Isolation and differentiation of phages from STEC strains after MC1061 lysogen infection

Strain	Culture filtrate PFUb	Primary phage isolated	PFU of secondary phagec	Secondary phage isolated	Ratio of primary to secondary phage isolate
E45040	1.23 × 105	φE45040-Stx2	0	None	NAd
E45040 (UV)a	8.34 × 108	φE45040-Stx2	0	None	NA
E83819	2.90 × 105	φE83819-Stx1	5.60 × 103	φE83819-Stx2	50:1
E85539	1.41 × 106	φE85539-Stx21	1.01 × 106	φE85539-Stx22	2:5
E86654	6.90 × 104	φE86654-Stx1	6.60 × 103	φE86654-Stx2	10:1
PS14	1.47 × 106	φPS14-Stx2	0	None	NA
PS14 (UV)	1.90 × 109	φPS14-Stx2	0	None	NA

^a(UV), culture was subjected to UV irradiation to induce phage production.

^bThat is the total amount of PFU found in the culture filtrates.

^cCalculated as the titer against MC1061 − the titer against the primary phage lysogen.

^dNA, not applicable.