TABLE 4.
Characterization of phage released from the double lysogens
| Double lysogena | Total PFU released (SEM) | LFU (SEM)b |
||
|---|---|---|---|---|
| φ24B::Kan | φ24B::Cat | φ24B::Kan φ24B::Cat | ||
| φ24B::Kan φ24B::Cat | ||||
| Uninduced | 8.0 × 105 (5.3 × 104) | 355 (45) | 0 (0) | 0 (0) |
| Induced | 2.1 × 108 (1.3 × 107) | 1,030 (141) | 673 (64) | 0 (0) |
| φ24B::Cat φ24B::Kan | ||||
| Uninduced | 7.6 × 105 (1.6 × 104) | 3 (3) | 0 (0) | 0 (0) |
| Induced | 2.6 × 108 (4.0 × 106) | 1,430 (57) | 837 (22) | 0 (0) |
a
Uninduced, phage spontaneously released after overnight culture of the double lysogen in LB medium plus Ca; induced phage released from the double lysogen after norfloxacin treatment (1 μg/ml, 1 h).
b
LFU, lysogen-forming units. Lysogens were detected after infection of naive MC1061 cells with culture filtrate from double-lysogen culture.