Table 2.
Genes essential for intracellular growth of F.tularensis SCHU S4 in Hep G2 cells
| Strain Name | Locus tag1/Genbank protein Accession # (*) | Gene Name (putative function) |
| Nucleotide metabolism | ||
| BJM1011 | FTT1663/YP_170570 | carA (carbamoyl-phosphate synthase small chain) |
| BJM1012 | FTT1664/YP_170571.1 | carB (carbamoyl-phosphate synthase large chain) |
| BJM1001 | FTT1665/YP_170572.1 (2X) | pyrB (aspartate carbamoyltransferase) |
| BJM1014 | FTT0204/YP_169264.1 | purA (adenylosuccinate synthetase) |
| BJM1015 | FTT1720c/YP_170622.1 (3X) | purL (phosphoribosylformylglycinamidine synthase) |
| BJM1018 | FTT0203c/YP_169263.1 (3X) | purH (bifunctional purine biosynthesis protein) |
| BJM1020 | FTT1721c/YP_170623.1 (2X) | purF (amidophosphoribosyltransferase)2 |
| Amino acid transport and metabolism | ||
| BJM1002 | FTT0588/YP_169611.1 | aroA (3-phosphoshikimate 1-carboxyvinyl transferase) |
| BJM1021 | FTT1181c/YP_170144.1 | ggt (gamma-glutamyl transpeptidase) |
| General function prediction | ||
| BJM1003 | FTT1244c/YP_170202.1 | yfiO (conserved hypothetical lipoprotein) |
| BJM1022 | FTT0129/YP_169196.1 | major facilitator super family (MFS) transport protein |
| BJM1004 | FTT0056c/YP_169131.1 | major facilitator super family (MFS) transport protein |
| Inorganic ion transport metabolism | ||
| BJM1024 | FTT1490/YP_170425.1 | Na+/H+ antiporter |
| Cell wall/membrane biogenesis | ||
| BJM1023 | FTT1234/YP_170192.1 | choloylglycine hydrolase family protein |
| BJM1026 | FTT1236/YP_170192.1 | hypothetical protein |
| BJM1028 | FTT1103/YP_170079.1 | conserved hypothetical lipoprotein |
| Lipid metabolism | ||
| BJM1013 | FTT1762c/YP_170653.1 | acetyltransferase protein |
| Posttranslational modification/protein turnover | ||
| BJM1025 | FTT1310c/YP_170263.1 | hflB (ATP-dependent metalloprotease) |
| BJM1005 | FTT0107c/YP_169177.1 (3X) | dsbB (disulfide bond formation protein) |
1Locus tag: http://biocyc.org/FRANT/
2This mutant was previously identified in F. tularensis novicida .
(*) Number of times a mutant in this ORF was isolated [11].