Table 3.
Invasion and intracellular survival of selected Schu S4 mutants in J774.11
| Strain | No. of intracellular CFUs recovered from cells at 24 hrs. |
| SCHU S4 | 3.00 × 107 ± 0.346 |
| BJM1025 (hflB) | 1.80 × 107 ± 0.200 p < 0.0065* |
| BJM1004 (MFS) | 1.35 × 107 ± 0.0866 p < 0.0013* |
| BJM1005 (dsbB) | 8.33 × 105 ± 0.0577 |
| BJM1026 (hypothetical) | 2.32 × 105 ± 1.29 |
| BJM1001 (pyrB) | 1.80 × 108 ± 0.0643 |
| BJM1002 (aroA) | 2.18 × 108 ± 0.387 |
| BJM1003 (yfiO) | 1.17 × 108 ± 0.135 |
| BJM10012 (carA) | 1.14 × 108 ± 0.867 |
| BJM10015 (purL) | 4.03 × 107 ± 0.802 |
| BJM10018 (purH) | 2.70 × 107 ± 0.100 |
| BJM1024 (Na+/H+ transporter) | 1.12 × 108 ± 0.872 |
1J774.1 cells were infected with bacteria at an MOI to 50:1 After 2 hrs the media was replaced with media containing gentamicin. Sets of three wells were lysed 24 hrs later, diluted, and spread on MHA plates to determine the number of intracellular colony forming units (CFUs). Each time point represents the average of three wells.
* Compared to Schu S4