Table 4.

Primer and MGB Taqman probe sequences and concentrations used in real time PCR.

Gene symbol	Primer/probe	Gene Sequence (5'-3')	Concentration (nM)
ACT1	Forward	TTTCATCTTCTGTATCAGAGGAACTTATTT	300
	Reverse	ATGGGATGAATCATCAAACAAGAG	300
	MGB probe	ATTATTTTCGTCACGGGTATT	300
RPP2B	Forward	TGCTTACTTATTGTTAGTTCAAGGTGGTA	300
	Reverse	CAACACCAACGGATTCCAATAAA	300
	MGB probe	CACCTCTCCATCAGCTT	200
TDH1 (GAPDH)	Forward	CGGTCCATCCCACAAGGA	300
	Reverse	AGTGGAAGATGGGATAATGTTACCA	300
	MGB probe	AGGTGGTAGAACTGC	200
PMA1	Forward	TTGCTTATGATAATGCTCCATACGA	300
	Reverse	TACCCCACAATCTTGGCAAGT	300
	MGB probe	CCAAAACCAGTTAAATGG	200
LSC2	Forward	CGTCAACATCTTTGGTGGTATTGT	900
	Reverse	TTGGTGGCAGCAATTAAACCT	900
	MGB probe	AGATGTGATTACGTCGCCA	400
CPA1	Forward	TCTGGTGTTGCTGCCATAACTG	300
	Reverse	AATTCTCCCCAATGATGAACCTT	300
	MGB probe	AGACAATTGGTGAGTTATT	200
IMH3	Forward	TATTCATATGGCATTATTGGGTGGTA	600
	Reverse	AACCATTTCTGCTTGTTCTTCAGA	600
	MGB probe	TGGTATCATTCATCATAACTGTAC	400
RIP	Forward	TGTCACGGTTCCCATTATGATATTT	300
	Reverse	TGGAATTTCCAAGTTCAATGGA	300
	MGB probe	TGGTAGAATTAGAAAGGGTCCA	200
ALS1	Forward	CAACAGGCACCTCAGCATCTAC	300
	Reverse	CTCCACCAGTAACAGATCCACTAGTAA	300
	MGB probe	AGTGCTAATAGCGAACTT	300
ALS3	Forward	CAACTTGGGTTATTGAAACAAAAACA	300
	Reverse	AGAAACAGAAACCCAAGAACAACCT	300
	MGB probe	AAACTAGCTGTGAAGGTGAT	300