FIGURE 6.
Data from representative assays of unregulated actin-activated Mg-NTPase activity of pcMyosin. (A) Linear time course of Pi release for ATP hydrolysis (100% ATP; solid circles) or dATP hydrolysis (100% dATP; open triangles). Conditions were as described in Methods. (B) Linear dependence of NTP hydrolysis rate on dATP/ATP ratio. Each point is derived from the slope of a time course of Pi production as shown in panel A (values from A correspond to the highest and lowest values on the abscissa). Lines represent linear least-squares regressions on the data.