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. 2006 Sep 1;2(9):e86. doi: 10.1371/journal.ppat.0020086

Figure 1. pTTSS Is Not Required for MLN Colonization.

Figure 1

BALB/c or C57BL6/J mice were intragastrically inoculated with 2 × 108 WT YPIII, YPIII yscBL, YPIII yscNU, YPIII yscBU, or YPIII pIB1−, and colonization levels of the ileum (A), (F), and (K), cecum (B), (G), and (L), PP (C), (H), and (M), MLN (D), (I), and (N), and spleen (E), (J), and (O) were determined at 6 h (A–E), 2 d (F–J), and 5 d (K–O) post-inoculation. BALB/c mice were intragastrically inoculated with 2 × 108 mouse commensal E. coli, and colonization levels of tissues were studied at 6 h (A–E) and 2 d post-inoculation (F–J). BALB/c mice were intragastrically inoculated with 2 × 109 of WT IP2666, IP2666 yscNU, or IP2666 pIB1−, and colonization levels were determined at 4 d post-inoculation ( [K–O], middle section).

Data are from 4–12 mice from at least two different experiments. All data was combined for each strain, tissue, and time point. Each square represents the log cfu/g tissue from one mouse; open squares indicate that less than 10 cfu were recovered per tissue, and bars represent the geometric mean. Asterisks (*) and black circles (•) indicate statistically significant differences between the WT and the mutants, calculated by the t test (*p < 0.01) or by Mann-Whitney (•U < 0.05), respectively.