Table 1.
Antibodies | Source | Antibody | HIER | Dilution | Staining interpretation |
Vimentin | DakoCytomation | Mo V9 | 2 | 1:50 | Cytoplasmic |
Cytokeratin | DakoCytomation | Mo AE1/AE3 | 1 | 1: 50 | Cytoplasmic |
S100 | DakoCytomation | Rabbit polyclonal | 1 | 1: 500 | Cytoplasmic +/-nuclear |
Desmin | DakoCytomation | Mo D33 | 2 | prediluted | Cytoplasmic |
SMA | DakoCytomation | Mo 1A4 | 2 | 1:50 | Cytopmasmic |
CD34 | Biogenex | Mo QBEnd 10 | 2 | Prediluted | Membranous |
CD10 | Novocastra | NCL-CD10-270 | 1 | 1:50 | Membranous |
Mo: mouse monoclonal.
1-Tris-EDTA pH9.0.
2-Citrate buffer pH6.0.