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G64S 3Dpol has a higher fidelity than WT 3Dpol in vitroa
| Nucleotide | Enzyme | Kd,app (μM) | kpol (s−1) | kpol/Kd,app (μM−1 s−1) | (kpol/Kd,app)WT/(kpol/Kd,app)G64S |
|---|---|---|---|---|---|
| ATP | WT | 130 ± 20 | 90 ± 5 | 0.65 | |
| G64S | 160 ± 10 | 30 ± 5 | 0.20 | 3 | |
| RTP | WT | 390 ± 40 | 1.0 ± 0.2 × 10−2 | 2.8 × 10−5 | |
| G64S | 370 ± 40 | 2.0 ± 0.2 × 10−3 | 5.7 × 10−6 | 5 | |
| GTP | WT | 330 ± 50 | 1.5 ± 0.2 × 10−2 | 4.5 × 10−5 | |
| G64S | 320 ± 50 | 3.5 ± 0.2 × 10−3 | 1.1 × 10−5 | 4 |
Experiments were performed as described in Experimental Procedures.
