Figure 1.

Phylogenetic tree of GLP proteins from barley and Arabidopsis. Multiple sequence alignment was carried out by using ClustalW with default parameter settings, including the Gonnet series of protein weight matrix (MegAlign software package; DNASTAR). Scale bar indicates amino acid substitutions. Boxed HvGER proteins correspond to genes selected for this study. TC numbers of Barley Gene Index at TIGR (release 9.0) corresponding to HvGER proteins are indicated, too. Only HvGER proteins represented by full CDS sequences are included in the multiple sequence alignment. Three partial cDNAs encoding additional putative HvGER4 paralogs were excluded from this analysis (TIGR TC139506, TC139507, and TC139508).