Figure 5.

Accumulation of recombinant HvGER proteins in transgenic Arabidopsis plants. Acid-soluble proteins were extracted from leaves of 10 T₀ plants (nos. 1–10) and used for western blotting. I, Extract from Bgh-inoculated wheat (Gf-2.8 blot) or barley (all other blots) leaves; C, extract from noninoculated wheat (Gf-2.8 blot) or barley (all other blots) leaves; wt, extract from Arabidopsis wild-type plants; H, high molecular mass complex (approximately 100-kD apparent molecular mass); M, monomeric protein band (approximately 22-kD apparent molecular mass). HvGER5a was detected by using a polyclonal serum directed against HvGER1a. Five micrograms of protein were loaded per lane.