Table IV.

Summary of functional characterization of HvGER genes with respect to the interaction of barley with B. graminis

n.a., Not analyzed.

Subfamily	Tested Genea	Experiment in Leaf	Induced by Bgh	Effect of Overepression	Effect of TIGS	OXOX Activityb	SOD Activityc	Enzymatic Activity Required for Resistance
HvGER1	HvGER1a	Inner tissues	Yes	None	n.a.	Yes	Nod	n.a.
HvGER2	HvGER2a	Epidermis and inner	No	Uncleare	None	No	No	Unclearf
HvGER3	HvGER3a	Epidermis	Yes	None	Resistance	No	n.a.	n.a.
HvGER4	HvGER4d	Epidermis	Yes	Resistance	Hypersusceptibility	No	Yes	Unclearg
HvGER5	HvGER5a	Epidermis	Yes	Resistance	Resistance	No	Yes	Yes
HvGER6	HvGER6a	None	No	None	n.a.	No	n.a.	n.a.

^aFor ID and accession number of cDNA or genomic clone corresponding to this gene, see Table I.

^bDetermined by transient expression in barley leaf epidermal cells.

^cDetermined by stable expression in transgenic Arabidopsis.

^dDetermined for wheat ortholog Gf-2.8 (germin gf-2.8 possessing OXOX activity; Schweizer et al., 1999b).

^eResistance-enhancing effect could not be reproduced in a second experimental series.

^fResistance-enhancing effect could not be reproduced in a second experimental series.

^gNot conclusive due to enhanced instability of recombinant mutant proteins in transgenic Arabidopsis.