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. 2006 Sep;142(1):364–376. doi: 10.1104/pp.106.084319

Figure 4.

Figure 4.

Role of the seventh and eighth positions in the TATA-box in light-dependent, lre-activated transcription from Pmec. A, GUS activities in 11-d-old light-grown (white bars) or dark-grown (black bars) seedlings containing lre-Pmec with the prototype TATA-box (lre-Pmec) and its variants with mutations A8C and T7G. The values above the bars indicate the sd from the means of three independent experiments. B, Semiquantitative RT-PCR carried out on five light-grown transgenic lines of lre-Pmec (lanes 3–7) and lre-Pmec-8C (lanes 8–12). Lanes 1 and 2 represent nontemplate control and semiquantitative RT-PCR of nontransformed tobacco, respectively. The values below the lanes indicate GUS activities in respective transgenic lines. The 26S rRNA levels detected by semiquantitative PCR are shown for comparison.