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. 2006 Sep;142(1):364–376. doi: 10.1104/pp.106.084319

Figure 6.

Figure 6.

Autoradiograph of 2% agarose (A) and 5% PAGE (B and C) showing nucleoprotein complex formation on 32P-labeled 120-bp Pmec oligo. A, In the presence of 0, 5, 10, 50, 100, 500, and 1,000 ng of NEL protein per 20 μL of incubation mixture (lanes 1–8). B, In the presence of 500 ng of NEL at 0-, 2-, 5-, 10-, 50-, and 100-fold molar excess of unlabeled 120-bp Pmec oligo (lanes 2–7). Lanes 8 to 11 show a supershift of the complex following addition of 0.5, 1.0, 2.0, and 5.0 μL of anti-TBP antibody. C, In the presence of 500 ng of NEL at 0-, 5-, 50-, 100-, and 500-fold molar excess (lanes 2–6) of unlabeled multimerized TATA-box sequence. Lane 1 shows the position of the free 32P-labeled 120-bp Pmec oligo.