Table I.

Synergistic effect of the nucleotides at different positions in the TATA-box on the specificity of promoter expression

GUS values are averages ± sd of data from three independent experiments comprising six to nine replicates each. The variation in GFP values expressed from the 35S promoter in the internal control were 34.05 ± 4.56 × 10³ RFU mg protein⁻¹.

Promoter Mutation	GUS Expression
		Dark	Light	Ratio (Dark/Light)
	103 pmol min−1 mg protein−1
Pmec	5.40 ± 1.10	5.00 ± 1.10	1.08 ± 0.22
A6→G	2.40 ± 0.45	6.49 ± 1.29	0.36 ± 0.06
A3→G	4.87 ± 1.11	1.52 ± 0.33	3.20 ± 0.62
T7→G	9.15 ± 1.92	0	∞
A8→C	2.60 ± 0.50	0	∞
A6→G; A3→G	0.48 ± 0.09	0	∞
A6→G; T7 →G	0	0	0
A6→G; A8→C	0	0	0