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Expressed proteins in E. coli, separated on SDS-PAGE, 20 μg protein/well. A, Melon UGGPase. B, Melon UGPase. C, Melon UT. For each enzyme, the three lanes represent, respectively, the MW marker, the E. coli extract with the expressed protein (+IPTG), and the E. coli extract without the heterologously expressed protein (−IPTG).
