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Comparison of substrate specificity of melon and tomato UGPase and UGGPase
Control noninduced transformed E. coli extracts (−IPTG) showed approximately 5% of the activity with either UDP-Gal or UDP-Glc, as compared to the induced (+IPTG) E. coli extracts. ND, Not detected.
| Substrates | Enzyme Activity
|
||
|---|---|---|---|
| Melon UGPase E. coli | Melon UGGPase E. coli | Tomato UGPase Native | |
| μmol mg protein−1 min−1 | |||
| UDP-Gal + PPi | 460 | 410 | 12 |
| UDP-Glc + PPi | 245 | 233 | 38 |
| Gal-1-P + UTP | ND | 678 | ND |
| Glc-1-P + UTP | 187 | 223 | 8 |
