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Dependence of Glc-1-P production from Gal-1-P and UTP on the addition of partially purified melon fruit UGGPase and purified epimerase
Each reaction was carried out with approximately 13 μg protein from fraction 18 of Figure 1A in a 1-mL reaction mix. ND, No activity detected.
| Substrate | Enzyme in Reaction
|
Glc-1-P Produced | |
|---|---|---|---|
| UGGPase | Epimerase | ||
| μmol Glc-1-P mg protein−1 min−1 | |||
| Gal-1-P, UTP | + | + | 1.8 |
| Gal-1-P, UTP | + | − | ND |
| Gal-1-P, UTP | − | − | ND |
