Table V.

Glc-1-P production from Gal-1-P and UTP with crude extracts of E. coli expressed protein of either melon UGGPase or melon UGPase, together with purified epimerase

The E. coli extract (−IPTG) did not express the UGGPase protein and served as blank reaction. Each reaction was carried out with approximately 10 μg protein from the crude E. coli extractions shown in Figure 3 in a 1-mL reaction mix. ND, No activity detected.

Substrate	E. coli Extract	Glc-1-P Produced
		μmol Glc-1-P mg protein−1 min−1
Gal-1-P, UTP	UGGPase, −IPTG	ND
Gal-1-P, UTP	UGGPase, +IPTG	14.5
Gal-1-P, UTP	UGPase, +IPTG	ND