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Identification of SUMOylated lysine residues within ScPCNA. ScPCNA (lane 1) and the ScPCNA protein variants K127/R (lane 2), K127/R + K164/R (lane 3), as well as K164/R (lane 4) were incubated with the Arabidopsis SUMOylation machinery and AtSUMO1. ScPCNA mobility shifts were detected by western-blot analysis using a Myc peptide-specific antiserum.
