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. 2006 Sep;142(1):318–332. doi: 10.1104/pp.106.085415

Figure 6.

Figure 6.

Analysis of AtSUMO2 multimerization by MS analysis and point mutagenesis. A, Structure of the branched AtSUMO2 peptide identified by LC-MS/MS. B, The Arabidopsis SUMO isoform AtSUMO2 labeled with an N-terminal HA tag (lane 1) and the thereof derived mutant variants K9/R (lane 2) and K10/R (lane 3) were incubated with all components of the Arabidopsis SUMOylation cascade and separated on a 15% SDS-polyacrylamide gel. AtSUMO2 mobility shifts were detected by western-blot analysis using a HA peptide-specific antiserum.