Figure 1.

A, Nucleotide and predicted amino acid sequences of mybys. The conserved R2/R3 DNA-binding domains at the 5′ end of the mybys (GenBank DQ311672) sequence are underlined and a typical activation domain at the 3′ end is in bold. The terminal codon is marked with an asterisk. B, Temporal and spatial regulation of mybys transcript levels in cucumber tissues. Total RNA extracted from cucumber leaves (L) and corollas at different developmental stages (stages 2–4) was probed with a radiolabeled fragment of mybys specific to the 3′ end of the gene. The same RNA blot was rehybridized with radiolabeled ChrC.