Figure 1.

Freezing exposure did not affect canola seed dry weight (A) or water content (B). At 20 DAP, canola plants were cooled 5°C/h until reaching −4°C, where the temperature was held for 6 h followed by rewarming at 5°C/h back to the growth temperature. The freeze-exposed plants were compared to control plants. The dry weight of the seeds was determined by incubating the seeds overnight at 75°C. Canola seed water content during development was calculated by (fresh weight − dry weight)/fresh weight. To separate the effects of freeze exposure from seed moisture loss on seed degreening, plants were maintained at high humidity during the freeze treatment and the recovery period.