FIG. 3.

IL-12 p40 production by in vitro-cultured pinnae and sdLN cells is sustained in vaccinated mice compared to that in infected mice. Skin biopsy samples and sdLN cells were obtained from naive mice and on days 2, 4, 8, and 14 after exposure to RA (solid) or normal larvae (hatched). Production of IL-12 p40 (a), IL-18 (b), and IL-10 (c) by pinnae at 18 h and production of IL-12 p40 (d) by sdLN at 72 h were determined in the absence of added antigen. IFN-γ (e) and IL-4 (f) production at 72 h was determined after stimulation with parasite antigen. Data are means + SEM for four to six pinnae and five sdLN. Statistical significance of differences between vaccinated and infected mice is shown. The horizontal dashed line shows dermal cytokine production in naive mice.