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Separation of pY-actin and unphosphorylated actin. (A) Elution profile of Mono P column of the actin sample shown in Fig. 3. (B) SDS/PAGE of fractions eluted between 60.5 and 70.5 ml stained with Coomassie blue (CB) and anti-actin and anti-pY antibodies. (C) Two-dimensional gel of pooled fractions of unphosphorylated actin (Actin) and P-actin and a 1:1 mixture of the purified proteins.
