Table 8.
Cytotoxicity of Fractioned Portions of the Frontier Herb Hp Soxhlet Ethanol Extracta
| HaCaT human keratinocytes % control survival(mean ±SE) | ||
|---|---|---|
| light | dark | |
| fraction 1 | ||
| 209 μg/mL | 41.9** (±1.8) | 44.9** (±3.0) |
| fraction 2 | ||
| 320 μg/mL | 19.1** (±3.9) | 23.1** (±4.0) |
| 160 μg/mL | 13.2** (±2.7) | 23.5** (±3.4) |
| fraction 3 | ||
| 194 μg/mL | 0.7** (±0.6) | 1.9** (±1.5) |
| 97 μg/mL | 4.8** (±2.0) | 9.9** (±5.9) |
| 48 μg/mL | 43.6** (±4.4) | 48.4** (±5.3) |
a
Cytotoxicity (% survival as compared to vehicle control-treated cells) of the C18 affinity column fractions derived from the Hp Soxhlet ethanol extract (n = 4–9). Fraction 1, 20% ACN elution; fraction 2, combined 27, 30, and 40% ACN elutions; and fraction 3, combined 50, 60, and 70% ACN elutions. Extracts were added to media at 1%, and 20 μM hypericin was used as the positive control, showing 8.4 (±4.3)% survival after light exposure and 73.5 (±2.2)% survival relative to the control in the dark, which are significantly different from each other.
**
= p < 0.0001 and
*
= p < 0.01 significantly different cell growth survival as compared to DMSO solvent control.