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. Author manuscript; available in PMC: 2006 Aug 30.
Published in final edited form as: Alcohol. 2005 Jun;36(2):83–90. doi: 10.1016/j.alcohol.2005.07.004

Figure 2.

Figure 2

Chronic ethanol ingestion differentially influences the apparent expression of some NMDA receptor subunit proteins. (A) Chronic ethanol exposure does not influence levels of total NR1 subunit protein or those subunits containing the C1 splice cassette (B). Examples of ‘typical’ western blots are shown at the top of each figure to illustrate the alternative loading of samples from control (‘C’) and chronic ethanol (‘E’) central amygdala. The summary of data obtained for each subunit is shown at the bottom of each figure. NMDA receptor subunit protein expression was normalized to the apparent levels of β-actin in each sample (see Methods). β-actin expression was not significantly affected by chronic ethanol exposure (see text). (C) Chronic ethanol ingestion modestly increases NR2A-like immunoreactivity in central nucleus samples. NR2A subunit protein was increased by ~20% compared to control central amygdala. ‘a’ – P~0.06, two-sided Student’s t-test. (D) Chronic ethanol exposure did not significantly influence the expression of NR2B-like immunoreactivity in the central amygdala.