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. Author manuscript; available in PMC: 2007 Jul 24.
Published in final edited form as: FEBS Lett. 2006 Jun 30;580(17):4131–4135. doi: 10.1016/j.febslet.2006.06.059

Figure 3.

Figure 3

Binding of MgATP (A) or MgAMPPNP (B) to the catalytic sites of F1 after preincubation with MgADP (open circles) or MgADP-fluoroaluminate (filled circles). Buffer was 50 mM Tris/H2SO4, 2.5 mM MgSO4, pH 8.0. The horizontal lines indicate the number of occupied sites after preincubation with 5 μM MgADP (dotted line) or 5 μM MgADP-fluoroaluminate (solid line), before addition of MgATP or MgAMPPNP. The x-axis scale represents the total Mg-nucleotide concentration, i.e. MgADP (± fluoroaluminate) and MgATP (A) or MgADP (± fluoroaluminate) and MgAMPPNP (B).