Endogenous co-expression of Alk5, type-II receptors, and Gdf11 in the facial primordia demonstrated by in situ hybridization. (A–C) Expression of Gdf11 in the tail and craniofacial primordia. Long arrowhead points to a strong signal in the mandibular arch at E10.5. (D–H) Whole-mount in situ hybridization with riboprobes for Alk5 (D), Acvr2A (E), Acvr2B (F), Bmpr2 (G), and Tgfbr2 (H). Arrowheads point to a positive signal in the nasal, maxillary and mandibular processes, second pharyngeal arch, and forebrain. ov—otic vesicle.