TABLE 3.
E. coli | Serogroup (intimin type) | No. of strains with the indicated PCR profile | PCR amplification (+) or no amplification (−) of:
|
||||
---|---|---|---|---|---|---|---|
ORF Z4326a | Efa1-5′b | Efa1-3′c | Efa1- Z4326d | EspF- Efa1e | |||
EHEC | O26 (β) | 1 | + | − | − | NDf | − |
O26 (β) | 1 | + | + | + | − | − | |
O26 (β) | 4 | + | + | + | + | + | |
O26 (β) | 6 | + | + | + | − | + | |
O45 (β) | 1 | + | + | + | + | − | |
O45 (ɛ) | 1 | + | + | + | + | − | |
O86 (γ) | 1 | + | − | − | ND | − | |
O103 (ɛ) | 3 | + | + | + | + | − | |
O111 (γ2) | 1 | + | + | + | − | − | |
O111 (γ2) | 1 | + | + | + | − | − | |
O111 (γ2) | 5 | + | + | + | + | − | |
O111 (γ2) | 1 | + | + | + | + | + | |
O118 (β) | 2 | + | + | + | + | − | |
O118 (β) | 1 | + | + | + | + | + | |
O121 (ɛ) | 1 | + | + | + | + | − | |
O123 (β) | 1 | + | + | + | − | + | |
O145 (γ1) | 2 | + | + | − | + | − | |
O145 (γ1) | 2 | + | + | + | + | +g | |
O157 (γ1) | 23h | + | + | − | + | − | |
EPEC | O26 (β) | 4 | + | + | + | + | + |
O26 (β) | 2 | + | + | + | + | − | |
O26 (β) | 2 | + | + | + | − | + | |
O26 (β) | 1 | + | + | + | − | − | |
O45 (β) | 1 | + | + | + | + | − | |
O55 (γ) | 2 | + | + | + | + | − | |
O55 (γ) | 2 | + | + | + | − | − | |
O55 (α) | 2 | + | + | + | − | − | |
O103 (β) | 2 | + | + | + | + | − | |
O111 (β) | 2 | + | + | + | + | − | |
O111 (β) | 1 | + | + | + | + | + | |
O114 (β) | 1 | + | + | + | + | + | |
O125 (α) | 2 | + | − | − | ND | − | |
O127 (γ) | 3 | + | − | − | ND | − | |
O127 (α) | 1 | + | + | + | − | − | |
O127 (α) | 1i | + | + | + | + | + | |
O128 (β) | 1 | + | − | − | ND | − | |
O128 (γ) | 2 | + | − | − | ND | − | |
O128 (β) | 1 | + | + | + | + | − | |
O128 (β) | 1 | + | + | + | − | + | |
ND (β) | 1 | + | + | + | + | + | |
ND (α) | 1 | + | + | + | + | + | |
ND (β) | 1 | + | + | + | + | − |
E in Fig. 1.
5′ Region of efa1/lifA (D in Fig. 1).
3′ Region of efa1/lifA (C in Fig. 1).
Linking region between the 5′ region of efa1/lifA and ORF Z4326 (B in Fig. 1).
Linking region between the LEE and PAI O#122 (A in Fig. 1).
ND, not determined.
The amplicon size was 4.5 kb.
Including reference strain EDL 933.
Reference strain E2348/69.