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. Author manuscript; available in PMC: 2007 Sep 1.
Published in final edited form as: J Pathol. 2006 Sep;210(1):59–66. doi: 10.1002/path.2000

Figure 4. CTGF/CCN2 protein expression in primary gingival epithelial and fibroblast cultures determined by Western blotting.

Figure 4

Western blots of cell layer extracts of primary human gingival epithelial cells (A) and human gingival fibroblasts (B) cultured and treated as described in “Methods and Materials” with 5 ng/ml TGF-β1, 10 μM LPA, or no treatment control. Blots analyzed with non-immune IgG gave no background signal. β-actin antibody was used to verify equal loading of gels. Blots are representative of four experiments with consistent results.