TABLE 2.
Reactivity of MAbs with recombinant fragments and synthetic peptides
| Fragment | Domaina (length [amino acids]) | MAb | Dodecapeptide | Hexapeptide(s)b | Epitope (sequence) |
|---|---|---|---|---|---|
| rOMP90-1 | 17S-87L (72) | EB3G2/IC4C7 | 41-TSEEFQVKETSS-52 | ND | 1 (TSEEFQVKETSSGT) |
| 43-EEFQVKETSSGT-54 | |||||
| p91Bf99a | 44E-89G (46) | JA6C7 | 52-SGAIYTCEGNVCISYAGK-69 | None | 2 (SGAIYTCEGNVCISYAGKDSPL) |
| 56-YTCEGNVCISYAGKDSPL-73 (2 × 18-mer, 91B) | |||||
| rOMP90-2 | 72G-137Y (65) | None | |||
| rOPM90-3 | 129Y-203D (74) | 73/0040 | None | ||
| 84/019 | None | ||||
| G11 | 152-SLVFHKNCSTAE-163 | ND | 3 (SLVFHKNCSTAE) | ||
| 191 | None | ||||
| 181 | 201-YADKLTIVSGGPT-212 | None | 4-(AIYADKLTIVSGGPTLFS) | ||
| rOPM90-4 | 193S-254N (61) | 73/0040 | None | ||
| 84/019 | None | ||||
| G11 | None | ||||
| 181 | 205-LTIVSGGPTLFS-216 | None | 4a (LTIVSGGPTLFS) | ||
| 191 | 225-SPKGGAISIKDS-236 | None | 5 (SPKGGAISIKDS) | ||
| rOPM90-5 | 244A-317I (73) | 73/04 | None | ||
| 192 | 249-ITFDGNKIIKTS-260 | None | 6 (ITFDGNKIIKTS) | ||
| FC1A2 | 281-LRAKDGFGIFFY-292 | None | 7 (LRAKDGFGIFFY) | ||
| 73/0200 | 281-LRAKDGFGIFFY-292 | DGFGIF, GFGIFF, | 7a (DGFGIF) | ||
| DA4E8 | 281-LRAKDGFGIFFY-292 | DGFGIF, GIFFYD, IFFYDP | 7a (DGFGIF) + 7b (GIFFYD) | ||
| 289-IFFYDPITGGGS-300 | |||||
| H11 | 289-IFFYDPITGGGS-300 | GIFFYD | 8 (IFFYDPITGGGS) | ||
| 73/0040 | 289-IFFYDPITGGGS-300 | None | 8-(IFFYDPITGGGS) | ||
| 73/0248 | 289-IFFYDPITGGGS-300 | GIFFYD, IFFYDP, FFYDPI, FYDPIT | 8a (FFYDPIT) | ||
| 73/053 | 289-IFFYDPITGGGS-300 | IFFYDF, FFYDPI, FYDPIT | 8a (FFYDPIT) | ||
| LB4B4 | 313-YTGKIVFSGEKL-324 | GKIVFS, KIVFSG, IVFSGE | 9 (GKIVFSGE) | ||
| rOPM90-6 | 301D-373M (72) | 73/0040 | None | ||
| LB4B4 | 313-YTGKIVFSGEKL-324 | GKIVFS, KIVFSG, IVFSGE | |||
| rOPM90-7 | 360A-431A (71) | 76/030 | 369-STVVMDLGTTLQ-380 | DLGTTL | 10 (DLGTTL) |
| 76/073 | 369-STVVMDLGTTLQ-380 | DLGTTL | |||
| 76/049 | 369-STVVMDLGTTLQ-380 | DLGTTL |
a
From start codon.
b
ND, not done. Bold indicates a strong reaction.