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RT-PCR analysis of C. albicans strains for TOK1 transcription. RT-PCR products of TOK1 transcripts in total RNA samples are displayed for three C. albicans strains: lane 1, CAI4 (TOK1/TOK1); lane 2, DBT2 (TOK1/tok1); and lane 3, DBT3 (tok1/tok1). Lane 4 resulted from using CAI4 genomic DNA as template. Fragments obtained by amplification with oligos specific for ACT1 (1.2 kb) and EFB1 (0.6 kb) (3) served as internal mRNA controls and demonstrated the cDNA origin.
