FIG. 5.

Disruption of TOK1 in C. albicans reduces sensitivity of cells to Hst 5 killing. C. allbicans strains were incubated at several concentrations of Hst 5, in 10 mM sodium phosphate buffer (pH 7.4) for 1.5 h at 37°C. Percent viability was calculated as the ratio of colonies recovered from Hst 5-treated cells/colonies recovered from control cells, multiplied by 100. Closed squares, CAI4 (TOK1/TOK1); open triangles, DBT2 (TOK1/tok1); closed circles, DBT3 (tok1/tok1). Each data point represents the mean ± standard deviation of at least three independent experiments. For a quantitative description, each of the plots was initially fitted with a simple saturation curve (rectangular hyperbola), but since all three half-saturation constants (K_0.5) were close together, the smooth curves shown were fitted using a common K_0.5, found to be 14.2 μM Hst 5.