Figure 12.

(A) Nucleotides that have been altered within in vivo and in vitro substrates the editing site NTrpoB C473. Underlined characters represent positions where substrates with sequence alterations have been assayed in vivo or in vitro. The asterisk indicates nucleotides that were changed in a substrate containing a −20 to +6 region of rpoB expressed in transplastomic plants by Reed et al. (19). Boldface characters represent nucleotides that when are altered within substrates are edited less efficiently than wild type. (B) Alignment of editing sites that contain S1, S2 and S3 sites. (C) Alignment of sequence of substrates containing 54 and 27 nt regions around NTrpoB C473 expressed in vivo and sequence from the substrates 3′ end II. Underlined characters represent regions of complementarity. (D) An alignment of sequence from the 3′ end I and wild-type sequence around the NTrpoB C473 site. Underlined sequences represent S1, S2 and S3 and similar sequences.