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. 2006 Aug 7;34(13):3762–3770. doi: 10.1093/nar/gkl545

Figure 1.

Figure 1

Sequence context of the mutable cytosines in the kan alleles. (A) The sequence of the plasmids used in this work around the proline codon (CCA or CCG) is shown. In plasmids pUP31, pUP41 and pUP44 the kan gene has the proline codon at position 94. The CCWGG site in pUP31 is underlined. The CCSGG sites in the different plasmids are indicated by green or brown boxes. (B) The effect of C to T mutation in codon 94 is shown. The mutations change a proline codon to either a leucine or serine codon and this restores kanamycin resistance.