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Sequence dependence of binding of PspGI to DNA. Gel electrophoretic mobility shift experiment to compare complex formation of PspGI and DNA with two different recognition sequences. Radioactively labeled PCR products containing either a ACCAGGC (A) or CCCGGGC (B) was incubated with various concentrations of PspGI (lanes 1–10). Free and bound DNA were separated by electrophoresis under non-denaturing conditions and detected using an instant imager.
