Fig. 3.

Analysis of ¹⁴C-labeled CHCl₃/CH₃OH (2:1) (A) and CHCl₃/CH₃OH/H₂O (10:10:3) (B) and LM/LAM fraction of M. smegmatis mc²155 (WT) (lanes 1) and ΔMSMEG4250 (mut) (lanes 2) cells (C). Cells were grown to an OD₆₀₀ of 0.4 and then incubated in the presence of 1 μCi/ml [¹⁴C]glucose for an additional 8 h. Cells were extracted with CHCl₃/CH₃OH (2:1) and CHCl₃/CH₃OH/H₂O (10:10:3) and analyzed by high-performance TLC. LM/LAM was extracted and analyzed by Tricine gel electrophoresis and subsequent blotting to a nitrocellulose membrane for autoradiography.