Table 1.
1H13C NMR chemical shift assignment based on 2D HSQC of LAM and mutLAM from WT and ΔMSMEG4250 cells
| Glycosyl residues | WT LAM |
mutLAM |
||||
|---|---|---|---|---|---|---|
| 13C | 1H | Volume | 13C | 1H | Volume | |
| 5-α-Araf | 110.0 | 5.18 | 83 | 109.8 | 5.19 | 95 |
| 5-α-Araf | 109.6 | 5.28 | 15 | 109.3 | 5.30 | 12 |
| 3,5-α-Araf | 110.3 | 5.13 | 347 | 110.1 | 5.11 | 392 |
| 2-α-Araf→5 | 108.6 | 5.19 | 75 | 108.4 | 5.20 | 79 |
| 2-α-Araf→3 | 108.4 | 5.26 | 19 | 108.1 | 5.27 | 25 |
| t-α-Araf→5 | — | — | — | 110.6 | 5.04 | 6 |
| t-α-Araf→3 | — | — | — | 109.7 | 5.25 | 4 |
| t-β-Araf | 103.5 | 5.16 | 100 | 103.3 | 5.17 | 100 |
| t-α-Manp | 105.1 | 5.07 | 37 | — | — | — |
| 2,6-α-Manp | 101.1 | 5.14 | 28 | — | — | — |
| 6-α-Manp | 102.4 | 4.93 | 8 | 102.0 | 4.92 | 93 |
Signal volumes were integrated and normalized to t-β-Araf, which was taken as the reference signal. Chemical shifts were measured in 2H2O at 500 MHz and 295 K. —, not detected.