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Southern blot hybridisation of EcoRI-digested genomic DNA from isolates of B. pseudomallei and B. thailandensis using oligonucleotide probe FR3. Lane M, λ-HindIII markers with molecular sizes (in kilobases) indicated on the left. Lane pMRG4 contains an 8.4 kb BclI fragment of the B. pseudomallei 204 genome spanning most of the mrgRS locus and 5 kb downstream, inserted in the phagemid pBK-CMV which was excised from λZAP Express and digested with EcoRI. See Table 1 for isolate details.
