Table 3.
Dilutions of primary and secondary antibodies for Western blotting
Primary Antibody | Dilution | Secondary Antibody | Dilution | Source |
Anti-MrgR | 1:500 | Swine Anti-rabbit IgG | 1:2000 | Bethyl Labs |
Anti-MrgS | 1:300 | Swine Anti-rabbit IgG | 1:1000 | Bethyl Labs |
Anti-MBP | 1:1000 | Swine Anti-rabbit IgG | 1:2000 | NEB |
Convalescent sera pooled from 6 melioidosis patients | 1:300 | Rabbit Anti-human IgG | 1:1000 | Ty Pitt, HPA, London |