Polypeptide sequence alignment of 42-kDa subunits
of Mg-chelatase. The barley sequence and 5 of the 14 sequences
available in the GenBank Database: Hvu (Hordeum vulgare;
barley; GenBank accession no. U26545), Gma
(Glycine max; soybean; D45857), Nta
(N. tabacum; tobacco; AF014053), Ath
(Arabidopsis thaliana; thale cress;
X91411), Rca (Rhodobacter capsulatus; Z11165), and Rsp
[R. sphaeroides; AF017642 (AJ001690)]. The N-terminal
sequence of the soybean, tobacco, and thale cress polypeptides is the
same as that of the unprocessed proteins. Conserved amino acid residues
in all 14 sequences are marked by asterisks. The species of origin of
the 42-kDa subunits (not displayed) are Porphyra
purpurea (U38804), Odontella sinensis (Z67753),
Cryptomonas phi (Z21976), Anabaena
variabilis (D49426), Synechocystis PCC6803
(U35144), Olisthodiscus luteus (Z21959),
Chlorobium vibrioforme (Z83933), and
Heliobacillus mobilis (AF080002). Arrows indicate the
amino acid residues changed in mutants: L91F, D187N, and R269K. The
tobacco Sulfur mutation is N269I.