Western blot analysis of the barley Mg-chelatase
subunits XANTHA-H (Top), XANTHA-F
(Middle), and XANTHA-G (Bottom). Lanes:
1, Chlorina-125; 2, Chlorina-157; 3,
Chlorina-161; 4, xantha-h57;
5, xantha-g28; 6,
xantha-f10; 7, wild-type chloroplast
high-speed supernatant; and 8, wild-type chloroplast high-speed pellet.
The molecular masses of the standard bands labeled in lanes 7 and 8 are
42 kDa for H, 150 kDa for F, and 76 kDa for G.